@article{10.1016/j.crmeth.2025.101113,
    author = {Alireza Saberigarakani and Riya P. Patel and Milad Almasian and Xinyuan Zhang and Jonathan Brewer and Sohail S. Hassan and Jichen Chai and Juhyun Lee and Baowei Fei and Jie Yuan and Kelli Carroll and Yichen Ding},
    title = {Volumetric imaging and computation to explore contractile function in zebrafish hearts},
    journal = {Cell Reports Methods},
    volume = {5},
    number = {8},
    pages = {101113},
    year = {2025},
    abstract = {Novel insights into cardiac contractile dysfunction at the cellular level could deepen understanding of arrhythmia and heart injury, which are leading causes of morbidity and mortality worldwide. We present a comprehensive experimental and computational framework combining light-field microscopy and single-cell tracking to investigate real-time volumetric data in live zebrafish hearts, which share structural and electrical similarities to the human heart. Our system acquires 200 vol/s with lateral resolution of up to 5.02 ± 0.54 μm and axial resolution of 9.02 ± 1.11 μm across the whole depth using an expectation-maximization-smoothed deconvolution algorithm. We apply a deep-learning approach to quantify cell displacement and velocity in blood flow and myocardial motion and to perform real-time volumetric tracking from end-systole to end-diastole within a virtual reality environment. This capability delivers high-speed and high-resolution imaging of cardiac contractility at single-cell resolution over multiple cycles, supporting in-depth investigation of intercellular interactions in health and disease.},
    doi = {10.1016/j.crmeth.2025.101113},
    url = {https://doi.org/10.1016/j.crmeth.2025.101113},
    eprint = {https://www.cell.com/cell-reports-methods/fulltext/S2667-2375(25)00149-3},
}